An alternative approach for gene transfer in trees using wild-type Agrobacterium strains.
Identifieur interne : 004C67 ( Main/Exploration ); précédent : 004C66; suivant : 004C68An alternative approach for gene transfer in trees using wild-type Agrobacterium strains.
Auteurs : A C Brasileiro [France] ; J C Leplé ; J. Muzzin ; D. Ounnoughi ; M F Michel ; L. JouaninSource :
- Plant molecular biology [ 0167-4412 ] ; 1991.
Descripteurs français
- KwdFr :
- ADN bactérien (biosynthèse), Arbres (MeSH), Glucuronidase (métabolisme), Hybridation d'acides nucléiques (MeSH), Kanamycin kinase (MeSH), Maladies des plantes (MeSH), Phosphotransferases (métabolisme), Plasmides (MeSH), Rhizobium (génétique), Techniques de culture (MeSH), Techniques génétiques (MeSH), Transformation génétique (MeSH).
- MESH :
- biosynthèse : ADN bactérien.
- génétique : Rhizobium.
- métabolisme : Glucuronidase, Phosphotransferases.
- Arbres, Hybridation d'acides nucléiques, Kanamycin kinase, Maladies des plantes, Plasmides, Techniques de culture, Techniques génétiques, Transformation génétique.
English descriptors
- KwdEn :
- Culture Techniques (MeSH), DNA, Bacterial (biosynthesis), Genetic Techniques (MeSH), Glucuronidase (metabolism), Kanamycin Kinase (MeSH), Nucleic Acid Hybridization (MeSH), Phosphotransferases (metabolism), Plant Diseases (MeSH), Plasmids (MeSH), Rhizobium (genetics), Transformation, Genetic (MeSH), Trees (MeSH).
- MESH :
- chemical , biosynthesis : DNA, Bacterial.
- chemical , metabolism : Glucuronidase, Phosphotransferases.
- genetics : Rhizobium.
- Culture Techniques, Genetic Techniques, Kanamycin Kinase, Nucleic Acid Hybridization, Plant Diseases, Plasmids, Transformation, Genetic, Trees.
Abstract
Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on walnut, gall formation was weak. On poplar and wild cherry, tumors induced by nopaline strains developed spontaneously shoots that had a normal phenotype and did not carry oncogenic T-DNA. From these observations, we have established a co-inoculation method to transform plants, using poplar as an experimental model. The method is based on inoculation of stem internodes with an Agrobacterium suspension containing both an oncogenic strain that induces shoot differentiation and a disarmed strain that provides the suitable genes in a binary vector. We used the vector pBI121 carrying neo (kanamycin resistance) and uidA (beta-glucuronidase) genes to facilitate early selection and screening. Poplar plants derived from kanamycin-resistant shoots that did not carry oncogenic T-DNA, were shown to contain and to express neo and uidA genes. These results suggest that wild-type Agrobacterium strains that induce shoot formation directly from tumors can be used as a general tool for gene transfer, avoiding difficult regeneration procedures.
DOI: 10.1007/BF00040638
PubMed: 1653060
Affiliations:
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Muzzin</name></author><author><name sortKey="Ounnoughi, D" sort="Ounnoughi, D" uniqKey="Ounnoughi D" first="D" last="Ounnoughi">D. Ounnoughi</name></author><author><name sortKey="Michel, M F" sort="Michel, M F" uniqKey="Michel M" first="M F" last="Michel">M F Michel</name></author><author><name sortKey="Jouanin, L" sort="Jouanin, L" uniqKey="Jouanin L" first="L" last="Jouanin">L. Jouanin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1991">1991</date><idno type="RBID">pubmed:1653060</idno><idno type="pmid">1653060</idno><idno type="doi">10.1007/BF00040638</idno><idno type="wicri:Area/Main/Corpus">004C47</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004C47</idno><idno type="wicri:Area/Main/Curation">004C47</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004C47</idno><idno type="wicri:Area/Main/Exploration">004C47</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">An alternative approach for gene transfer in trees using wild-type Agrobacterium strains.</title><author><name sortKey="Brasileiro, A C" sort="Brasileiro, A C" uniqKey="Brasileiro A" first="A C" last="Brasileiro">A C Brasileiro</name><affiliation wicri:level="3"><nlm:affiliation>Laboratoire de Biologie Cellulaire, INRA, Versailles, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Biologie Cellulaire, INRA, Versailles</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Versailles</settlement></placeName></affiliation></author><author><name sortKey="Leple, J C" sort="Leple, J C" uniqKey="Leple J" first="J C" last="Leplé">J C Leplé</name></author><author><name sortKey="Muzzin, J" sort="Muzzin, J" uniqKey="Muzzin J" first="J" last="Muzzin">J. Muzzin</name></author><author><name sortKey="Ounnoughi, D" sort="Ounnoughi, D" uniqKey="Ounnoughi D" first="D" last="Ounnoughi">D. Ounnoughi</name></author><author><name sortKey="Michel, M F" sort="Michel, M F" uniqKey="Michel M" first="M F" last="Michel">M F Michel</name></author><author><name sortKey="Jouanin, L" sort="Jouanin, L" uniqKey="Jouanin L" first="L" last="Jouanin">L. Jouanin</name></author></analytic><series><title level="j">Plant molecular biology</title><idno type="ISSN">0167-4412</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Culture Techniques (MeSH)</term><term>DNA, Bacterial (biosynthesis)</term><term>Genetic Techniques (MeSH)</term><term>Glucuronidase (metabolism)</term><term>Kanamycin Kinase (MeSH)</term><term>Nucleic Acid Hybridization (MeSH)</term><term>Phosphotransferases (metabolism)</term><term>Plant Diseases (MeSH)</term><term>Plasmids (MeSH)</term><term>Rhizobium (genetics)</term><term>Transformation, Genetic (MeSH)</term><term>Trees (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (biosynthèse)</term><term>Arbres (MeSH)</term><term>Glucuronidase (métabolisme)</term><term>Hybridation d'acides nucléiques (MeSH)</term><term>Kanamycin kinase (MeSH)</term><term>Maladies des plantes (MeSH)</term><term>Phosphotransferases (métabolisme)</term><term>Plasmides (MeSH)</term><term>Rhizobium (génétique)</term><term>Techniques de culture (MeSH)</term><term>Techniques génétiques (MeSH)</term><term>Transformation génétique (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term><term>Phosphotransferases</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ADN bactérien</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glucuronidase</term><term>Phosphotransferases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Culture Techniques</term><term>Genetic Techniques</term><term>Kanamycin Kinase</term><term>Nucleic Acid Hybridization</term><term>Plant Diseases</term><term>Plasmids</term><term>Transformation, Genetic</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arbres</term><term>Hybridation d'acides nucléiques</term><term>Kanamycin kinase</term><term>Maladies des plantes</term><term>Plasmides</term><term>Techniques de culture</term><term>Techniques génétiques</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on walnut, gall formation was weak. On poplar and wild cherry, tumors induced by nopaline strains developed spontaneously shoots that had a normal phenotype and did not carry oncogenic T-DNA. From these observations, we have established a co-inoculation method to transform plants, using poplar as an experimental model. The method is based on inoculation of stem internodes with an Agrobacterium suspension containing both an oncogenic strain that induces shoot differentiation and a disarmed strain that provides the suitable genes in a binary vector. We used the vector pBI121 carrying neo (kanamycin resistance) and uidA (beta-glucuronidase) genes to facilitate early selection and screening. Poplar plants derived from kanamycin-resistant shoots that did not carry oncogenic T-DNA, were shown to contain and to express neo and uidA genes. These results suggest that wild-type Agrobacterium strains that induce shoot formation directly from tumors can be used as a general tool for gene transfer, avoiding difficult regeneration procedures.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">1653060</PMID><DateCompleted><Year>1991</Year><Month>10</Month><Day>09</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0167-4412</ISSN><JournalIssue CitedMedium="Print"><Volume>17</Volume><Issue>3</Issue><PubDate><Year>1991</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Plant molecular biology</Title><ISOAbbreviation>Plant Mol Biol</ISOAbbreviation></Journal><ArticleTitle>An alternative approach for gene transfer in trees using wild-type Agrobacterium strains.</ArticleTitle><Pagination><MedlinePgn>441-52</MedlinePgn></Pagination><Abstract><AbstractText>Micropropagated shoots of three forest tree species, poplar (Populus tremula x P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra x J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on walnut, gall formation was weak. On poplar and wild cherry, tumors induced by nopaline strains developed spontaneously shoots that had a normal phenotype and did not carry oncogenic T-DNA. From these observations, we have established a co-inoculation method to transform plants, using poplar as an experimental model. The method is based on inoculation of stem internodes with an Agrobacterium suspension containing both an oncogenic strain that induces shoot differentiation and a disarmed strain that provides the suitable genes in a binary vector. We used the vector pBI121 carrying neo (kanamycin resistance) and uidA (beta-glucuronidase) genes to facilitate early selection and screening. Poplar plants derived from kanamycin-resistant shoots that did not carry oncogenic T-DNA, were shown to contain and to express neo and uidA genes. These results suggest that wild-type Agrobacterium strains that induce shoot formation directly from tumors can be used as a general tool for gene transfer, avoiding difficult regeneration procedures.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Brasileiro</LastName><ForeName>A C</ForeName><Initials>AC</Initials><AffiliationInfo><Affiliation>Laboratoire de Biologie Cellulaire, INRA, Versailles, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leplé</LastName><ForeName>J C</ForeName><Initials>JC</Initials></Author><Author ValidYN="Y"><LastName>Muzzin</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Ounnoughi</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Michel</LastName><ForeName>M F</ForeName><Initials>MF</Initials></Author><Author ValidYN="Y"><LastName>Jouanin</LastName><ForeName>L</ForeName><Initials>L</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Plant Mol Biol</MedlineTA><NlmUniqueID>9106343</NlmUniqueID><ISSNLinking>0167-4412</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C036483">T-DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.-</RegistryNumber><NameOfSubstance UI="D010770">Phosphotransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.1.95</RegistryNumber><NameOfSubstance UI="D019868">Kanamycin Kinase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D046508" MajorTopicYN="N">Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004269" MajorTopicYN="N">DNA, Bacterial</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005821" MajorTopicYN="N">Genetic Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019868" MajorTopicYN="N">Kanamycin Kinase</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009693" MajorTopicYN="N">Nucleic Acid Hybridization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010770" MajorTopicYN="N">Phosphotransferases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1991</Year><Month>9</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1991</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1991</Year><Month>9</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">1653060</ArticleId><ArticleId IdType="doi">10.1007/BF00040638</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1983 Jan;80(2):407-11</Citation><ArticleIdList><ArticleId IdType="pubmed">16593270</ArticleId></ArticleIdList></Reference><Reference><Citation>Plasmid. 1980 Mar;3(2):193-211</Citation><ArticleIdList><ArticleId IdType="pubmed">6100893</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1990 Jul;93(3):1110-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16667565</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 1990 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Jouanin</name><name sortKey="Leple, J C" sort="Leple, J C" uniqKey="Leple J" first="J C" last="Leplé">J C Leplé</name><name sortKey="Michel, M F" sort="Michel, M F" uniqKey="Michel M" first="M F" last="Michel">M F Michel</name><name sortKey="Muzzin, J" sort="Muzzin, J" uniqKey="Muzzin J" first="J" last="Muzzin">J. Muzzin</name><name sortKey="Ounnoughi, D" sort="Ounnoughi, D" uniqKey="Ounnoughi D" first="D" last="Ounnoughi">D. Ounnoughi</name></noCountry><country name="France"><region name="Île-de-France"><name sortKey="Brasileiro, A C" sort="Brasileiro, A C" uniqKey="Brasileiro A" first="A C" last="Brasileiro">A C Brasileiro</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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